Journal: Experimental and Therapeutic Medicine

Article Title: Application of an acellular dermal matrix to a rabbit model of oral mucosal defects

doi: 10.3892/etm.2018.5705

Figure Lengend Snippet: Expression of VEGF in the control and ADM groups. (A) Expression of VEGF mRNA determined using reverse transcription-quantitative polymerase chain reaction. (B) Western blot analysis of VEGF protein. (C) Quantification of western blot analysis. Data are expressed as mean ± standard deviation of three repeated experiments. *P<0.05. VEGF, vascular endothelial growth factor; ADM, acellular dermal matrix.

Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 12% SDS-PAGE, transferred onto PVDF membranes (EMD Millipore, Billerica, MA, USA) and blocked with 10% bovine serum albumin (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) at room temperature for 2 h. Membranes were then incubated with antibodies specific to GLUT1 (1:1,000; cat no. NB110-39113; Novus Biologicals Canada ULC, Oakville, ON, Canada), vascular endothelial growth factor (VEGF) (1:500; cat no. MAB293R; R&D Systems, Inc., Minneapolis, MN, USA) and GAPDH (1:2,000; cat no. GTX100118; GeneTex, Inc., Irvine, CA, USA) overnight at 4°C, followed by incubation with anti-rabbit Immunoglobulin G horseradish peroxidase-conjugated antibodies (1:5,000; cat no. 7071; Cell Signaling Technology, Inc., Danvers, MA, USA) for 2 h at room temperature.

Techniques: Expressing, Control, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation